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Fix NEST/AGenBio trough reservoir well geometry #1045

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Merged
BioCam merged 1 commit into from
May 18, 2026
Merged

Fix NEST/AGenBio trough reservoir well geometry #1045

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27 changes: 17 additions & 10 deletions pylabrobot/resources/agenbio/plates.py
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Original file line number Diff line number Diff line change
Expand Up @@ -136,18 +136,19 @@ def AGenBio_1_wellplate_Fl(name: str, lid: Optional[Lid] = None) -> Plate:


def AGenBio_1_troughplate_190000uL_Fl(name: str, lid: Optional[Lid] = None) -> Plate:
"""
AGenBio Catalog No. RES-190-F
- Material: Polypropylene
- Max. volume: 190 mL
"""AGenBio single-well reagent reservoir, 190 mL, flat-bottom (ANSI/SLAS footprint).

cat. no.: RES-190-F
Material: polypropylene
"""
INNER_WELL_WIDTH = 107.2 # measured
INNER_WELL_HEIGHT = 70.9 # measured

well_kwargs = {
"size_x": INNER_WELL_WIDTH, # measured
"size_y": INNER_WELL_HEIGHT, # measured
"size_z": 24.76, # measured to bottom of well
"size_z": 44.2 - 5.88, # well cavity reaches the plate top (plate size_z 44.2 - dz 5.88)
"max_volume": 190_000, # spec rating; size_z reaches the rim so the box volume would overstate capacity
"bottom_type": WellBottomType.FLAT,
"cross_section_type": CrossSectionType.RECTANGLE,
"compute_height_from_volume": lambda liquid_volume: compute_height_from_volume_rectangle(
Expand Down Expand Up @@ -185,25 +186,31 @@ def AGenBio_1_troughplate_190000uL_Fl(name: str, lid: Optional[Lid] = None) -> P


def AGenBio_1_troughplate_100000uL_Fl(name: str, lid: Optional[Lid] = None) -> Plate:
"""
AGenBio Catalog No. RES-100-F
- Material: Polypropylene
- Max. volume: 100 mL
"""AGenBio single-well reagent reservoir, 100 mL, flat-bottom (ANSI/SLAS footprint).

cat. no.: RES-100-F
Material: polypropylene
"""
INNER_WELL_WIDTH = 107.2 # measured
INNER_WELL_HEIGHT = 70.9 # measured

well_kwargs = {
"size_x": INNER_WELL_WIDTH, # measured
"size_y": INNER_WELL_HEIGHT, # measured
"size_z": 13, # measured to bottom of well
"size_z": 31.4 - 5.88, # well cavity reaches the plate top (plate size_z 31.4 - dz 5.88)
"max_volume": 100_000, # spec rating; size_z reaches the rim so the box volume would overstate capacity
"bottom_type": WellBottomType.FLAT,
"cross_section_type": CrossSectionType.RECTANGLE,
"compute_height_from_volume": lambda liquid_volume: compute_height_from_volume_rectangle(
liquid_volume,
INNER_WELL_HEIGHT,
INNER_WELL_WIDTH,
),
"compute_volume_from_height": lambda liquid_height: compute_volume_from_height_rectangle(
liquid_height,
INNER_WELL_HEIGHT,
INNER_WELL_WIDTH,
),
"material_z_thickness": 1,
}

Expand Down
26 changes: 21 additions & 5 deletions pylabrobot/resources/nest/plates.py
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Original file line number Diff line number Diff line change
Expand Up @@ -54,16 +54,22 @@ def nest_1_troughplate_195000uL_Vb(name: str) -> Plate:


def nest_1_troughplate_185000uL_Vb(name: str) -> Plate:
"""part no 360104. 384 tiny holes, but one container."""
"""NEST single-trough reagent reservoir plate, 185 mL, V-bottom (ANSI/SLAS footprint).

cat. no.: 360104

384 tiny holes feed one shared container.
"""
real_well_d = (85.48 - 8.99 * 2) / 15 # 4.5. in the drawing it says 2.4 which is wrong

well_size_y = 127.76 - (12.13 - real_well_d / 2) * 2 # from datasheet
well_size_x = 85.48 - (8.99 - real_well_d / 2) * 2 # from datasheet
well_size_x = 127.76 - (12.13 - real_well_d / 2) * 2 # from datasheet
well_size_y = 85.48 - (8.99 - real_well_d / 2) * 2 # from datasheet
well_kwargs = {
"size_x": well_size_x,
"size_y": well_size_y,
"size_z": 26.85, # from datasheet
"bottom_type": WellBottomType.V,
"cross_section_type": CrossSectionType.RECTANGLE,
# an approximation: the trapezoid at the bottom is not fully defined in the datasheet
"compute_height_from_volume": lambda liquid_volume: compute_height_from_volume_rectangle(
liquid_volume=liquid_volume, well_length=well_size_x, well_width=well_size_y
Expand Down Expand Up @@ -96,14 +102,20 @@ def nest_1_troughplate_185000uL_Vb(name: str) -> Plate:


def nest_8_troughplate_22000uL_Vb(name: str) -> Plate:
"""part no 360101. not validated"""
"""NEST 8-trough reagent reservoir plate, 22 mL, V-bottom (ANSI/SLAS footprint).

cat. no.: 360101

Not validated.
"""
well_size_x = 107.5 # from datasheet
well_size_y = 8.2 # from datasheet
well_kwargs = {
"size_x": well_size_x,
"size_y": well_size_y,
"size_z": 26.85, # from datasheet
"bottom_type": WellBottomType.V,
"cross_section_type": CrossSectionType.RECTANGLE,
# an approximation: the trapezoid at the bottom is not fully defined in the datasheet
"compute_height_from_volume": lambda liquid_volume: compute_height_from_volume_rectangle(
liquid_volume=liquid_volume, well_length=well_size_x, well_width=well_size_y
Expand Down Expand Up @@ -136,14 +148,18 @@ def nest_8_troughplate_22000uL_Vb(name: str) -> Plate:


def nest_12_troughplate_15000uL_Vb(name: str) -> Plate:
"""part no 360102."""
"""NEST 12-trough reagent reservoir plate, 15 mL, V-bottom (ANSI/SLAS footprint).

cat. no.: 360102
"""
well_size_x = 8.2 # from datasheet
well_size_y = 71.2 # from datasheet
well_kwargs = {
"size_x": well_size_x,
"size_y": well_size_y,
"size_z": 26.85, # from datasheet
"bottom_type": WellBottomType.V,
"cross_section_type": CrossSectionType.RECTANGLE,
# an approximation: the trapezoid at the bottom is not fully defined in the datasheet
"compute_height_from_volume": lambda liquid_volume: compute_height_from_volume_rectangle(
liquid_volume=liquid_volume, well_length=well_size_x, well_width=well_size_y
Expand Down
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