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27 changes: 27 additions & 0 deletions kb/communities/Kombucha_KMC_IMBG1_Fermentation_Community.yaml
Original file line number Diff line number Diff line change
Expand Up @@ -207,4 +207,31 @@ associated_datasets:
evidence_source: IN_VITRO
snippet: submitted to the GenBank database under an accession numbers KF908872-KF90879
explanation: Supports the associated GenBank sequence-accession range reported by the study.
related_ingredients:
- preferred_term: sucrose
chebi_term:
id: CHEBI:17992
label: sucrose
relevance: >
Sucrose is the primary fermentable carbon source added to the black-tea substrate that sustains
the KMC-IMBG1 yeast and acetobacterial community.
evidence:
- reference: PMID:26061774
supports: SUPPORT
evidence_source: IN_VITRO
snippet: black tea (Lipton, 1.2%, w/v) extract with sucrose (3.0%, w/v)
explanation: Names sucrose as the sweetener added to the black-tea fermentation medium.
- preferred_term: cellulose
chebi_term:
id: CHEBI:18246
label: (1->4)-beta-D-glucan
relevance: >
Cellulose is produced by the acetobacterial members and forms the surface pellicle that physically
structures the kombucha community.
evidence:
- reference: doi:10.1186/s13568-015-0124-5
supports: SUPPORT
evidence_source: IN_VITRO
snippet: cellulose-based pellicles created by the cellulose-producing bacteria
explanation: Names cellulose as the pellicle material produced by community bacteria.
metal_relevance: NOT_APPLICABLE
Original file line number Diff line number Diff line change
Expand Up @@ -238,6 +238,72 @@ environmental_factors:
evidence_source: IN_VITRO
snippet: from air, water, phosphate, trace metals, and sunlight
explanation: Supports trace metals as reported inorganic inputs.
related_ingredients:
- preferred_term: sucrose
chebi_term:
id: CHEBI:17992
label: sucrose
relevance: >
Sucrose is the cross-fed carbon currency: engineered cscB S. elongatus exports sucrose, and the
coculture cannot grow without a sucrose-exporting S. elongatus partner.
evidence:
- reference: PMID:27232890
supports: SUPPORT
evidence_source: IN_VITRO
snippet: sucrose-exporting S. elongatus
explanation: Names sucrose export by S. elongatus as required for coculture growth.
- preferred_term: polyhydroxybutyrate
chebi_term:
id: CHEBI:53389
label: poly(3-hydroxybutyrate)
relevance: >
Polyhydroxybutyrate (PHB) is the industrially relevant biopolymer the designed coculture was shown
to produce from simple inorganic inputs and light.
evidence:
- reference: PMID:27232890
supports: SUPPORT
evidence_source: IN_VITRO
snippet: polyhydroxybutyrate (PHB) and alginate
explanation: Names polyhydroxybutyrate as an industrially relevant product of the system.
- preferred_term: alginate
chebi_term:
id: CHEBI:58187
label: alginate
relevance: >
Alginate is named as a second industrially relevant biopolymer the coculture has the potential to
produce alongside PHB.
evidence:
- reference: PMID:27232890
supports: SUPPORT
evidence_source: IN_VITRO
snippet: polyhydroxybutyrate (PHB) and alginate
explanation: Names alginate as a potential industrially relevant product of the coculture.
- preferred_term: glutamate
chebi_term:
id: CHEBI:18237
label: glutamic acid
relevance: >
Glutamate was supplied in the initial coculture studies that established the cross-feeding model
between S. elongatus and A. vinelandii.
evidence:
- reference: PMID:27232890
supports: SUPPORT
evidence_source: IN_VITRO
snippet: media with glutamate
explanation: Names glutamate as a medium component in the initial cross-feeding studies.
- preferred_term: phosphate
chebi_term:
id: CHEBI:18367
label: phosphate(3-)
relevance: >
Phosphate is one of the simple inorganic inputs from which the coculture concept was designed to
drive chemical photoproduction.
evidence:
- reference: PMID:27232890
supports: SUPPORT
evidence_source: IN_VITRO
snippet: from air, water, phosphate, trace
explanation: Names phosphate among the simple inorganic inputs for photoproduction.
growth_media:
- name: Light-driven Synechococcus-Azotobacter coculture
composition:
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Original file line number Diff line number Diff line change
Expand Up @@ -232,5 +232,20 @@ associated_datasets:
url: https://doi.org/10.1186/s13068-017-0736-x
description: Open-access study reporting growth, sucrose use, ROS, biomass, and lipid measurements for
S. elongatus-yeast artificial lichen cocultures.
related_ingredients:
- preferred_term: sucrose
chebi_term:
id: CHEBI:17992
label: sucrose
relevance: >
Sucrose is the central exchanged metabolite of this synthetic lichen coculture: engineered
cscB+ Synechococcus elongatus secretes it as photosynthate and Rhodotorula glutinis consumes it
as its carbon source for growth and lipid production.
evidence:
- reference: doi:10.1186/s13068-017-0736-x
supports: SUPPORT
evidence_source: IN_VITRO
snippet: sucrose-secreting cyanobacterium Synechococcus elongatus
explanation: Names sucrose as the metabolite secreted by the engineered cyanobacterial partner.
metal_relevance: NOT_APPLICABLE

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