diff --git a/sentry-android-core/src/main/java/io/sentry/android/core/AndroidContinuousProfiler.java b/sentry-android-core/src/main/java/io/sentry/android/core/AndroidContinuousProfiler.java
index 41362c9d93..a26b836332 100644
--- a/sentry-android-core/src/main/java/io/sentry/android/core/AndroidContinuousProfiler.java
+++ b/sentry-android-core/src/main/java/io/sentry/android/core/AndroidContinuousProfiler.java
@@ -146,6 +146,7 @@ public void startProfiler(
           break;
       }
       if (!isRunning()) {
+        shouldStop = false;
         logger.log(SentryLevel.DEBUG, "Started Profiler.");
         start();
       }
diff --git a/sentry-android-core/src/test/java/io/sentry/android/core/AndroidContinuousProfilerTest.kt b/sentry-android-core/src/test/java/io/sentry/android/core/AndroidContinuousProfilerTest.kt
index 162e56c36e..70dc651951 100644
--- a/sentry-android-core/src/test/java/io/sentry/android/core/AndroidContinuousProfilerTest.kt
+++ b/sentry-android-core/src/test/java/io/sentry/android/core/AndroidContinuousProfilerTest.kt
@@ -556,6 +556,24 @@ class AndroidContinuousProfilerTest {
       .log(eq(SentryLevel.WARNING), eq("Device is offline. Stopping profiler."))
   }
 
+  @Test
+  fun `manual profiler can be started again after a full start-stop cycle`() {
+    val profiler = fixture.getSut()
+
+    profiler.startProfiler(ProfileLifecycle.MANUAL, fixture.mockTracesSampler)
+    assertTrue(profiler.isRunning)
+    profiler.stopProfiler(ProfileLifecycle.MANUAL)
+    // Triggers the scheduled executor task that collects perf metrics and finalizes the chunk
+    fixture.executor.runAll()
+    assertFalse(profiler.isRunning)
+
+    profiler.startProfiler(ProfileLifecycle.MANUAL, fixture.mockTracesSampler)
+    assertTrue(profiler.isRunning)
+    // Triggers the scheduled executor task that collects perf metrics and finalizes the chunk
+    fixture.executor.runAll()
+    assertTrue(profiler.isRunning, "shouldStop must be reset on start")
+  }
+
   fun withMockScopes(closure: () -> Unit) =
     Mockito.mockStatic(Sentry::class.java).use {
       it.`when`<Any> { Sentry.getCurrentScopes() }.thenReturn(fixture.scopes)