diff --git a/mysql-test/main/ps_mem_leaks.result b/mysql-test/main/ps_mem_leaks.result
index fa4f7e36ed967..df1285fb2ee63 100644
--- a/mysql-test/main/ps_mem_leaks.result
+++ b/mysql-test/main/ps_mem_leaks.result
@@ -367,4 +367,25 @@ a
 # Clean up
 DEALLOCATE PREPARE stmt;
 DROP TABLE t1;
+#
+# MDEV-39265: Assertion `(mem_root->flags & 4) == 0` failed upon 2nd execution `USING DEFAULT` with sequence
+#
+CREATE SEQUENCE s;
+CREATE TABLE t (a INT DEFAULT(NEXTVAL(s)));
+INSERT INTO t VALUES (1), (2);
+PREPARE stmt FROM "UPDATE t SET a = ?";
+# Verify no SIGABRT
+EXECUTE stmt USING 3;
+EXECUTE stmt USING DEFAULT;
+# Verify the there are no repeated allocations on statement mem_root
+SET @saved_dbug = @@SESSION.debug_dbug;
+SET SESSION debug_dbug="+d,assert_no_alloc_internal_tables";
+EXECUTE stmt USING DEFAULT;
+EXECUTE stmt USING 4;
+EXECUTE stmt USING DEFAULT;
+EXECUTE stmt USING DEFAULT;
+SET SESSION debug_dbug= @saved_dbug;
+DEALLOCATE PREPARE stmt;
+DROP SEQUENCE s;
+DROP TABLE t;
 # End of 10.6 tests
diff --git a/mysql-test/main/ps_mem_leaks.test b/mysql-test/main/ps_mem_leaks.test
index 3374519908fda..ef67f13659c34 100644
--- a/mysql-test/main/ps_mem_leaks.test
+++ b/mysql-test/main/ps_mem_leaks.test
@@ -364,4 +364,34 @@ EXECUTE stmt USING 4;
 DEALLOCATE PREPARE stmt;
 DROP TABLE t1;
 
+--echo #
+--echo # MDEV-39265: Assertion `(mem_root->flags & 4) == 0` failed upon 2nd execution `USING DEFAULT` with sequence
+--echo #
+
+CREATE SEQUENCE s;
+CREATE TABLE t (a INT DEFAULT(NEXTVAL(s)));
+INSERT INTO t VALUES (1), (2);
+
+PREPARE stmt FROM "UPDATE t SET a = ?";
+
+--echo # Verify no SIGABRT
+EXECUTE stmt USING 3;
+EXECUTE stmt USING DEFAULT;
+
+--echo # Verify the there are no repeated allocations on statement mem_root
+
+SET @saved_dbug = @@SESSION.debug_dbug;
+SET SESSION debug_dbug="+d,assert_no_alloc_internal_tables";
+
+EXECUTE stmt USING DEFAULT;
+EXECUTE stmt USING 4;
+EXECUTE stmt USING DEFAULT;
+EXECUTE stmt USING DEFAULT;
+
+SET SESSION debug_dbug= @saved_dbug;
+
+DEALLOCATE PREPARE stmt;
+DROP SEQUENCE s;
+DROP TABLE t;
+
 --echo # End of 10.6 tests
diff --git a/sql/sql_base.cc b/sql/sql_base.cc
index 79d7d7d32324a..e2c6015bcec88 100644
--- a/sql/sql_base.cc
+++ b/sql/sql_base.cc
@@ -4762,7 +4762,29 @@ add_internal_tables(THD *thd, Query_tables_list *prelocking_ctx,
       continue;
     }
 
+    /*
+      Debug hook: Verify we only allocate once per internal table.
+    */
+     DBUG_EXECUTE_IF("assert_no_alloc_internal_tables", { DBUG_ASSERT(0); });
+
+    /*
+      When a prepared statement uses DEFAULT (like sequence tables) in its
+      second or further execution AND if the table is not already on statement's
+      mem_root, temporarily allow allocating on statement mem_root.
+    */
+#ifdef PROTECT_STATEMENT_MEMROOT
+    const bool read_only_mem_root= (thd->mem_root->flags & ROOT_FLAG_READ_ONLY);
+    if (read_only_mem_root)
+      thd->mem_root->flags&= ~ROOT_FLAG_READ_ONLY;
+#endif
+
     TABLE_LIST *tl= (TABLE_LIST *) thd->alloc(sizeof(TABLE_LIST));
+
+#ifdef PROTECT_STATEMENT_MEMROOT
+    if (read_only_mem_root)
+      thd->mem_root->flags|= ROOT_FLAG_READ_ONLY;
+#endif
+
     if (!tl)
       DBUG_RETURN(TRUE);
     tl->init_one_table_for_prelocking(&tables->db,